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To report your yield as a percent yield, you need to know how many units of enzyme activity you have after this step and how many units of enzyme activity you started with. Suppose that you want to compare the amount of activity after the dialysis step to the total amount of enzyme that was originally present in the crude extract. In this case you divide the number of units in the dialysate by the total number of units in the crude extract and multiply by 100%. This is the percent yield of enzyme activity. Suppose that you have 100,000 units of enzyme activity in the crude extract and you have 25,000 units left by the time you get to the dialysis step.
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