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Yield is the total amount of product that you have. For enzymes, yield is best expressed in total units of enzymatic activity so you would use the results of your enzyme assay to determine the yield. Yield will decrease as the purification proceeds because losses are inevitable as you manipulate the extract. Yield is not expressed as a concentration because this does not tell you the total amount you obtained. For example, If you are a strawberry farmer and you have 18 bushels of strawberries per row, what would be your strawberry yield if you have 50 rows of strawberries planted? If you report the yield as 18 bushels per row, it has no meaning since no one knows how many rows you have. It is the same idea with enzyme activity. Do not report the yield of enzyme activity in units/ml since no one knows whether you have 1000 ml or 0.25 ml.
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